Title:
【発明の名称】巨大分子及び細胞の分画用のマイクロリソグラフ配列
Document Type and Number:
Japanese Patent JPH09504362
Kind Code:
A
Abstract:
A sorting apparatus and method for fractionating and simultaneously viewing individual microstructures, such as free cells, viruses, macromolecules, or minute particles in a fluid medium. The sorting apparatus is composed of a substrate having a receptacle located therein, the receptacle having sidewalls and a floor. An array of obstacles is positioned within the receptacle with the obstacles upstanding from the floor of the receptacle. A transparent cover overlies the array of obstacles to cover the receptacle and afford visual observation of migration of the microstructures exclusively through the array of obstacles. Electrodes may be positioned within the receptacle to generate an electric field in the fluid medium in the receptacle in order to induce the migration of the microstructures. Migration of the microstructures may also occur, for example, by a hydrodynamic field, an optical field, a magnetic field, or a gravity field applied to the receptacle. The obstacles of the array of obstacles may be of various shapes such as round posts, rectangular bunkers, or v-shaped or cup-shaped structures. The arrays of obstacles are formed of a predetermined and reproducible pattern, and can be reused. Methods for manufacturing and using the apparatus are also claimed.
Inventors:
Robert Hamilton, Austin
Wayne David, Volkmas
Russ Van, Lynn Charles
Wayne David, Volkmas
Russ Van, Lynn Charles
Application Number:
JP50193995A
Publication Date:
April 28, 1997
Filing Date:
June 03, 1994
Export Citation:
Assignee:
British Technology Group USA Inc.
International Classes:
B01J19/00; B01L3/00; C12M1/42; C12M3/08; G01N27/447; G01N15/02; G01N15/14; G01N30/60; G01N30/92; (IPC1-7): G01N27/447; C12M1/42; G01N27/447
Attorney, Agent or Firm:
Kyozo Yuasa (6 people outside)