Title:
【発明の名称】新規生物活性のスクリーニング
Document Type and Number:
Japanese Patent JP2001514017
Kind Code:
A
Abstract:
Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing an assay system. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
Inventors:
Short, Jay, M.
Application Number:
JP2000507845A
Publication Date:
September 11, 2001
Filing Date:
August 26, 1998
Export Citation:
Assignee:
Diversa corporation
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12N15/10; C12N15/52; C12P19/34; C12Q1/00; C12Q1/02; C12Q1/25; C12Q1/68; G01N33/50; G01N33/566; (IPC1-7): C12Q1/68; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12P19/34; G01N33/50; G01N33/566
Attorney, Agent or Firm:
Yusuke Hiraki (1 person outside)