Title:
【発明の名称】ヒト多能性幹細胞の増殖および分化のための技術
Document Type and Number:
Japanese Patent JP2003530828
Kind Code:
A
Abstract:
This disclosure provides an improved system for culturing human pluripotent stem (pPS) cells in the absence of feeder cells. The role of the feeder cells can be replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium. Permanent cell lines are provided that can produce conditioned medium on a commercial scale. Methods have also been discovered to genetically alter pPS cells by introducing the cells with a viral vector or DNA/lipid complex. The system described in this disclosure allows for bulk proliferation of pPS cells for use in studying the biology of pPS cell differentiation, and the production of important products for use in human therapy.
Inventors:
Carpenter Melisa, K.
Funk Walter, Dee.
Gold Joseph, Dee.
Inokuma Margaret, S.
Shu Chun Hui
Funk Walter, Dee.
Gold Joseph, Dee.
Inokuma Margaret, S.
Shu Chun Hui
Application Number:
JP2001551190A
Publication Date:
October 21, 2003
Filing Date:
January 10, 2001
Export Citation:
Assignee:
GERON CORPORATION
International Classes:
C12N5/02; C12N5/07; C12N15/09; C12N5/073; C12N5/0735; C12N5/077; C12N5/079; C12N5/0793; C12N5/10; C12N15/10; C12P21/02; C12Q1/02; A61K35/12; C12R1/91; (IPC1-7): C12N15/09; C12N5/06; C12P21/02
Attorney, Agent or Firm:
Shimizu Hatsushi (1 person outside)