【発明の名称】インフルエンザ菌B型の外膜タンパク質P1およびペプチド類

Japanese Patent JP2644625

B2

The gene for outer membrane protein P1 of Haemophilus influenzae b is expressed in E. coli. Methods for expression and demonstration of the immunogenicity of recombinant P1 and portions thereof are disclosed, along with an improved method for the purification of P1. The nucleotide sequence of the P1 gene and the derived amino acid sequence of the P1 protein of Haemophilus influenzae type b are disclosed and the methods used to determine the same. Also disclosed are the methods used to clone and express the P1 gene as well as the purification protocol for the P1 gene products (recombinant P1 and P1 fusion proteins). Fourteen peptides are synthesized corresponding to specific sequences of the mature P1 protein. The use of the P1 protein as n immunogens for immunization against the disease caused by Haemophilus influenzae type b and the use of the protein as a carrier for conjugation with an oligosaccharide derived from Haemophilus to generate a potentially efficacious vaccine against the disease, are described. Also disclosed is the use of P1 peptide-conjugates as immunizing agents to elicit anti-Haemophilus influenzae type b antibodies.

MANSON ROBAATO ESU JUNIA
GURAASU SUUZAN
CHON PEIREI
YAN YANNPIN
FUAHIMU RAAFUATO
SHIIA DOO YAN CHAARUZU
MATSUKUERII PATORITSUKU
KUREIN MAIKERU

JP51468090A

August 25, 1997

October 31, 1990

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KONNOOTO LAB LTD
WASHINTON UNIV

A61K39/00; A61K39/102; A61K39/385; A61P31/04; A61P31/12; A61P31/16; C07K14/005; C07K14/195; C07K14/285; C07K14/41; C07K14/705; C07K19/00; C12N15/09; C12N15/31; C12P21/02; G01N33/569; C12R1/19; C12R1/21; (IPC1-7): C07K14/285

JP63190896A

Ｉｎｔｅｃｔｉｏｎ ａｎｄ Ｉｍｍｕｎｉｔｙ，５６［９］（１９８８）Ｐ．２２３５−２２４２

Wakabayashi Tadashi

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