Title:
【発明の名称】インターロイキン-3(IL-3)多重変異ポリペプチド
Document Type and Number:
Japanese Patent JPH08503706
Kind Code:
A
Abstract:
The present invention relates to recombinant human interleukin-3 (hIL-3) variant or mutant proteins (muteins). These hIL-3 muteins contain one or more amino acid substitutions and may also have amino acid deletions at both the N- and C- termini. The invention also relates to pharmaceutical compositions containing the hIL-3 muteins and methods for using them. Additionally, the present invention relates to recombinant expression vectors comprising nucleotide sequences encoding the hIL-3 muteins, related microbial expression systems, and processes for making the hIL-3 muteins using the microbial expression systems. Included in the present invention are deletion mutants of hIL-3 in which from 1 to 14 amino acids have been deleted from the N-terminus, and from 1 to 15 amino acids (corresponding to residues 119 to 133) have been deleted from the C-terminus, and which also contain one to three amino acid substitutions in the polypeptide. These hIL-3 mutant polypeptides may have biological activities similar to or better than hIL-3 and, in some cases, may also have an improved side effect profile.
Inventors:
Bauer, S. Christopher
Abrams, Mark Allen
Braford-Goldberg Salrus
Caparon, Meir Helena
Alan Mickle, Easton
Crane, Barbara Quar
McKean, John Patrick
Orleans, Peter Oh.
Pike, Kumnan
Thomas, John Warren
Abrams, Mark Allen
Braford-Goldberg Salrus
Caparon, Meir Helena
Alan Mickle, Easton
Crane, Barbara Quar
McKean, John Patrick
Orleans, Peter Oh.
Pike, Kumnan
Thomas, John Warren
Application Number:
JP51324894A
Publication Date:
April 23, 1996
Filing Date:
November 22, 1993
Export Citation:
Assignee:
G.D.Searle & Company
International Classes:
A61K38/00; A61K38/20; A61K45/00; A61P7/00; A61P43/00; C07H21/04; C07K1/00; C07K14/00; C07K14/54; C07K17/00; C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/08; C12N5/10; C12N15/24; C12N15/61; C12P21/02; C12P21/04; C12R1/19; (IPC1-7): C07K14/54; A61K38/00; C07H21/04; C12N1/21; C12N15/09; C12P21/02
Attorney, Agent or Firm:
Akira Asamura (3 outside)