Title:
【発明の名称】結合モチーフを同定するための環状ペプチドライブラリーおよびその使用の方法
Document Type and Number:
Japanese Patent JP2002503231
Kind Code:
A
Abstract:
Methods for determining an optimal binding motif for a binding compound are provided in which the binding compound is contacted with an oriented degenerate cyclic peptide library (ODCPL) under conditions which allow for interaction between the binding compound and the ODCPL such that a complex is formed between the binding compound and a subpopulation of library members capable of interacting with the binding compound. The subpopulation of library members capable of interacting with the binding compound is then separated from library members that are incapable of interacting with the binding compound. The subpopulation of library members capable of interacting with the binding compound is linearized to form a subpopulation of linearized library members. The amino acid sequence of the subpopulation of linearized library members is determined and an amino acid sequence motif is then determined for an interaction site of the binding compound, based upon the relative abundance of different amino acid residues at each degenerate position within the linearized library members. Oriented degenerate cyclic peptide libraries, and methods for purifying cyclic peptides from linear peptides, are also provided.
Inventors:
Rye, Han-Sen
Yaffy, Michael Bee.
Song Yang, Elephant
Callaway, Kermit El., The Third
Cantley, Ruwissey.
Yaffy, Michael Bee.
Song Yang, Elephant
Callaway, Kermit El., The Third
Cantley, Ruwissey.
Application Number:
JP50090499A
Publication Date:
January 29, 2002
Filing Date:
May 28, 1998
Export Citation:
Assignee:
Beth Israel Dekanes Medical Center, Incorporated
International Classes:
C07K1/04; C07K4/00; C07K7/64; C07K16/00; C12Q1/37; C12Q1/42; C12Q1/48; G01N33/68; (IPC1-7): C07K4/00; C07K16/00; C12Q1/37; C12Q1/42; C12Q1/48; G01N33/68
Attorney, Agent or Firm:
Shusaku Yamamoto