Title:
【発明の名称】デンプン合成酵素をコードするdull1、およびその使用
Document Type and Number:
Japanese Patent JP2001522604
Kind Code:
A
Abstract:
The maize gene dull1 (du1) of the present invention is a determinant of the structure of endosperm starch. Mutations of du1 affect the activity of at least two enzymes involved in starch biosynthesis, namely the starch synthase, SSII, and the starch branching enzyme, SBEIIa. Du1 codes for a predicted 1674 residue protein, and is expressed with a unique temporal pattern in endosperm but is undetectable in leaf or root. The size of the Du1 product and its expression pattern match precisely the known characteristics of maize SSII. The Du1 product contains two different repeated regions in its unique amino terminus, one of which is identical to a conserved segment of the starch debranching enzymes. The cDNA provided for in the present invention encodes SSII, and mutations within this gene affect multiple aspects of starch biogenesis by disrupting an enzyme complex containing starch synthase(s), starch branching enzyme(s), and possibly starch debranching enzyme(s).
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Inventors:
Myers, Alan, M.
James, Martha, Gee.
James, Martha, Gee.
Application Number:
JP2000520569A
Publication Date:
November 20, 2001
Filing Date:
November 12, 1998
Export Citation:
Assignee:
Iowa State University Research Foundation, Inc.
International Classes:
A01H5/00; B01J4/02; C07H21/04; C12N5/10; C12N9/00; C12N9/10; C12N15/09; C12N15/29; C12N15/82; C12N15/84; C12P19/04; (IPC1-7): C12N15/09; A01H5/00; C12N5/10; C12N9/00; C12P19/04
Attorney, Agent or Firm:
Akimoto Teruo
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