Title:
3-ケトステロイド9-α-ヒドロキシラーゼ遺伝子の同定および3-ケトステロイド9-α-ヒドロキシラーゼ活性を遮断された微生物
Document Type and Number:
Japanese Patent JP2005517443
Kind Code:
A
Abstract:
The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence encoding the amino acid sequence of KshA protein or of KshB protein, encoded by nucleotides 499-1695 of SEQ ID NO:1 or by nucleotides 387-1427 of SEQ ID NO:2, respectively, and functional homologues thereof. The polynucleotides of the invention can be used to construct genetically modified microorganisms blocked in 3-ketosteroid 9alpha-hydroxylase activity, which are useful in the microbial degradation of steroids to accumulate certain steroid products.
Inventors:
Juan Del Heise, Roberto
Juan del Maiden, Peter
Hetsel, Gerda
Dyke Heisen, Liu's Belt
Juan del Maiden, Peter
Hetsel, Gerda
Dyke Heisen, Liu's Belt
Application Number:
JP2003569818A
Publication Date:
June 16, 2005
Filing Date:
February 19, 2003
Export Citation:
Assignee:
Akzo Novel N B
International Classes:
C12N15/09; C12N1/21; C12N9/02; C12P33/00; C12P33/18; (IPC1-7): C12N15/09; C12N1/21; C12N9/02; C12P33/18
Attorney, Agent or Firm:
Yoshio Kawaguchi
Akio Ichiiri
Makoto Ono
Katsuma Osaki
Mitsuaki Tsubokura
Akio Ichiiri
Makoto Ono
Katsuma Osaki
Mitsuaki Tsubokura