PURPOSE: To obtain the titled globulin containing albumin in a given amount based on the immunoglobulin and no flocculated immunoglobulin in high purity and yield, by treating a supernatant liquid separated from blood plasma with ethanol under specific conditions.
CONSTITUTION: A supernatant liquid separated from blood plasma is treated with ethanol at 5.5W6.5 pH in 30W35% ethanolic concentration and 1.0W2.0% proteinic concentration at 0.08 ionic strength to give a supernatant liquid containing albumin and a precipitate of immunoglobulin containing 15W20%, based on the immunoglobulin, albumin. Distilled water is added to the resultant precipitate, and ethanol is then added to the formed dispersion to give 10W16% ethanolic concentration, and the formed precipitate is removed by the centrifugation to afford a supernatant liquid. Ethanol is then added to the resultant supernatant liquid to give 20W25% ethanol concentration and remove the formed precipitate. The pH is further adjusted to 4.5W5.0, and the ethanolic concentration is kept at 20W25%. The same procedures are repeated once more to increase the ionic strength to about 0.1, separate the precipitate and give the aimed immunoglobulin pharmaceutical for the intravenous injection.
KUROSU FUMIO
INOUCHI TOSHITSUGU
KOMATSU MASAKATA
