Title:
免疫グロブリン変異体
Document Type and Number:
Japanese Patent JP4836147
Kind Code:
B2
Abstract:
A method for making a humanized antibody comprising amino acid sequence of a non-human, import antibody and a human antibody, comprising the steps of: a. obtaining the amino acid sequences of at least a portion of an import variable domain and of a consensus human variable domain; b. identifying Complementarity Determining Region (CDR) amino acid sequences in the import and the human variable domain sequences; c. substituting an import CDR amino acid sequence for the corresponding human CDR amino acid sequence; d. aligning the amino acid sequences of a Framework Region (FR) of the import antibody and the corresponding FR of the consensus antibody; e. identifying import antibody FR residues in the aligned FR sequences that are non-homologous to the corresponding consensus antibody residues; f. determining if the non-homologous import amino acid residue is reasonably expected to have at least one of the following effects: 1. non-covalently binds antigen directly; 2. interacts with a CDR; or 3. participates in the VL- VH interface; and g. for any non-homologous import antibody amino acid residue which is reasonably expected to have at least one of these effects, substituting that residue for the corresponding amino acid residue in the consensus antibody FR sequence.
Inventors:
Carter, Paul Jay
Presta, Leonard G
Presta, Leonard G
Application Number:
JP2008158861A
Publication Date:
December 14, 2011
Filing Date:
June 18, 2008
Export Citation:
Assignee:
Genentech, Incorporated
International Classes:
C07K16/00; C07K16/46; A61K39/395; A61P35/00; C07K14/00; C07K16/18; C07K16/28; C07K16/32; C07K19/00; C12N15/09; C12N15/13; C12P21/08; G06F15/00; A61K38/00; C12P21/02; C12R1/19; C12R1/91
Foreign References:
| WO1990007861A1 |
Other References:
Proc. Natl. Acad. Sci. USA, Vol.86, p.10029-10033 (1989)
Proc. Natl. Acad. Sci. USA, Vol.88, p.2869-2873 (April 1991)
Nature, Vol.332, p.323-327 (1988)
J. Mol. Biol., Vol.196, p.901-917 (1987)
Nature, Vol.342, p.877-883 (1989)
Cancer Res., Vol.50, p.1550-1558 (1990)
Proc. Natl. Acad. Sci. USA, Vol.88, p.2869-2873 (April 1991)
Nature, Vol.332, p.323-327 (1988)
J. Mol. Biol., Vol.196, p.901-917 (1987)
Nature, Vol.342, p.877-883 (1989)
Cancer Res., Vol.50, p.1550-1558 (1990)
Attorney, Agent or Firm:
Hajime Tsukuni
Suzuki Otoya
Suzuki Otoya