Title:
IMPROVED ANALYTICAL METHOD
Document Type and Number:
Japanese Patent JPS5221891
Kind Code:
A
Abstract:
Method of assaying thyroid hormone in blood samples in which the sample is mixed with pepsin at pH 1, it is incubated at a suitable temp. for sufficient time to digest the protein attached to the thyroid hormone, the pH is adjusted to a value at which pepsin is inactive, then assaying the liberated thyroid hormone. Elimination of serum and plasma proteins, which bind the hormone, was only partially successful by earlier techniques. After pepsin digestion, the thyroid hormone is completely liberated.
Inventors:
HARORUDO BEITSU
Application Number:
JP9571975A
Publication Date:
February 18, 1977
Filing Date:
August 05, 1975
Export Citation:
Assignee:
CENTER LABORATORY MEDICINE
International Classes:
G01N33/74; C12Q1/37; G01N33/78; (IPC1-7): G01N31/14; G01N31/22; G01N33/16