Title:
基質に結合した特異的核酸結合剤のハイブリダイゼーション速度の向上
Document Type and Number:
Japanese Patent JP2005520568
Kind Code:
A
Abstract:
The invention relates to kits and methods for hybridizing nucleic acids with a specific nucleic acid-binding agent, such as a complementary nucleic acid. Previously, others have hybridized a nucleic acid with such an agent bound to a substrate. The improved methods described herein comprise binding a polycationizable attractor compound to the substrate, in addition to the agent. Examples of suitable polycationizable attractor compounds include polypeptides, including those with tunable cationizable amino acid residues, such as histidine. Compositions, kits, devices, and methods that make use of this hybridization rate enhancement technology are disclosed.
Inventors:
Kelly Be Gunning
Tom por drill
Michael Hogan
Tom por drill
Michael Hogan
Application Number:
JP2003580491A
Publication Date:
July 14, 2005
Filing Date:
March 21, 2003
Export Citation:
Assignee:
Sigma-Genosys Limited Partnership
International Classes:
C12N15/09; C07K5/078; C07K5/097; C07K5/117; C12M1/00; C12Q1/68; C40B40/06; C40B40/10; (IPC1-7): C12Q1/68; C12M1/00; C12N15/09
Attorney, Agent or Firm:
Kyousei Tamura
Hiromitsu Takayama
Minato Saito
Hiromitsu Takayama
Minato Saito