Title:
誘導型高発現系
Document Type and Number:
Japanese Patent JP4180862
Kind Code:
B2
Abstract:
The present invention provides a vector containing a DNA construct containing a first promoter that is a nitrilase gene promoter, a multicloning site and a nitrilase transcription regulatory protein gene (particularly preferably a structural gene is ligated into a multicloning site and a second promoter is placed at the upstream of the nitrilase transcription regulatory protein gene), and the like. The present invention affords a high expression system capable of expressing a large amount of useful proteins by actinomycete host represented by actinomycete belonging to the genus Streptomyces and the like beneficially used for the production of useful proteins.
Inventors:
Tatsuhiko Kobayashi
Yoshiteru Hashimoto
East end Hiroki
Yoshiteru Hashimoto
East end Hiroki
Application Number:
JP2002260679A
Publication Date:
November 12, 2008
Filing Date:
September 05, 2002
Export Citation:
Assignee:
Tatsuhiko Kobayashi
Astellas Pharma Inc.
Astellas Pharma Inc.
International Classes:
C12N15/09; C07H21/04; C12N1/21; C12N9/78; C12N15/74
Domestic Patent References:
| JP9028380A |
Other References:
Proc. Natl. Acad. Sci. USA,1996年,Vol.93,pp.10572-10577
Current Opinion in Biotechnology,2001年,Vol.12,pp.446-449
Trends in Biotechnology,1997年,Vol.15,pp.315-320
Current Opinion in Biotechnology,2001年,Vol.12,pp.446-449
Trends in Biotechnology,1997年,Vol.15,pp.315-320
Attorney, Agent or Firm:
Takashima Ichi