Title:
阻害オリゴヌクレオチド及び遺伝子を特異的に抑制するためのそれらの使用
Document Type and Number:
Japanese Patent JP4323952
Kind Code:
B2
Abstract:
Double-stranded oligonucleotides (I) consisting of two complementary strands (Ia; Ib), each having at one of their 3' and 5' ends 1-5 unpaired nucleotides, forming single-stranded overhangs, are new. One of (Ia) and (Ib) is complementary to a target sequence, of DNA or RNA, that is to be specifically repressed. ACTIVITY : Cytostatic; Virucide; Anti-HIV; Neuroprotective; Ophthalmological; Antidiabetic; Antipsoriatic; Antirheumatic; Antiarthritic. The oligonucleotide hybrid (A) prepared from sequences of formulae ggggaggcagccauugagacTT gucucaauggcugccuccccTT (lower case indicates ribonucleotides and upper case deoxyribonucleotides) was designed to target the junction of the PML/RARalpha fusion gene associated with promyelocytic leukemia. It (500 ng) was introduced into NIH 3T3 cells, co-transfected with a vector (100 ng) that expressed the PML/RARalpha fusion. Western blotting analysis after 48 hours showed greatly reduced expression of the fusion protein, relative to a control without (A). MECHANISM OF ACTION : Antisense inhibition of gene expression.
Inventors:
Annick Harrell-Belan
Surimanuai-Shi-Ari
Florence Caboon-Jet Jet
Anne Shaw Shelow
Francois Dautri
Louis Martinez
Surimanuai-Shi-Ari
Florence Caboon-Jet Jet
Anne Shaw Shelow
Francois Dautri
Louis Martinez
Application Number:
JP2003542613A
Publication Date:
September 02, 2009
Filing Date:
November 08, 2002
Export Citation:
Assignee:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE(CNRS)
International Classes:
C12N15/09; A61K31/7105; A61K31/711; A61K31/713; A61K48/00; A61P5/28; A61P9/10; A61P9/14; A61P17/06; A61P19/02; A61P25/00; A61P27/02; A61P29/00; A61P31/00; A61P31/12; A61P31/18; A61P35/00; A61P43/00; C12N15/113; A61K38/00
Foreign References:
| WO2001036646A1 | ||||
| WO2001075164A1 | ||||
| WO1994018835A1 | ||||
| US5556956 | ||||
| WO2001052904A1 |
Other References:
Nature,2001年 5月,Vol.411,p.494-498
Genes and Development,2001年 1月,Vol.15,p.188-200
Gynecologic Oncology,1997年,Vol.66,p.94-102
Jananese Journal of Cancer Research,1996年,Vol.87, No.7,p.735-742
Blood,1996年,Vol.88, No.4,p.1411-1417
Genes and Development,2001年 1月,Vol.15,p.188-200
Gynecologic Oncology,1997年,Vol.66,p.94-102
Jananese Journal of Cancer Research,1996年,Vol.87, No.7,p.735-742
Blood,1996年,Vol.88, No.4,p.1411-1417
Attorney, Agent or Firm:
Masago Onishi
Nobuyuki Yamaguchi
Nobuyuki Yamaguchi