Title:
指示された補足
Document Type and Number:
Japanese Patent JP4686598
Kind Code:
B2
Abstract:
A method of producing a tumorigenic mouse cell, the tumorigenicity of which depends on a recombinant gene of interest is disclosed. The method involves: (a) providing a conditionally tumorigenic mouse cell containing a recombinant oncogene operably linked to an inducible promoter, wherein (i) expression of the recombinant oncogene is necessary and sufficient for the tumorigenicity of the tumorigenic mouse cell, and (ii) the inducible promoter is in the uninduced state; and (b) introducing into the cell a recombinant gene of interest that functionally complements the oncogene. Also disclosed is a method of testing a compound for anti-tumor effects. The method includes producing tumorigenic mouse cells the tumorigenicity depends on expression of a recombinant gene of interest, implanting the cells in mice and obtaining tumors from the implanted cells, administering test compounds to the mice, and determining anti-tumor effects, if any, of the compounds.
Inventors:
Robinson, Murray
O'Hagan, Ronan
Canaan, Caluppier
Kai, Chi
Chiu, Maria Isabel
Learner, Lorina
Rin, Chie
Butterfly, inhoi
O'Hagan, Ronan
Canaan, Caluppier
Kai, Chi
Chiu, Maria Isabel
Learner, Lorina
Rin, Chie
Butterfly, inhoi
Application Number:
JP2008505554A
Publication Date:
May 25, 2011
Filing Date:
April 05, 2006
Export Citation:
Assignee:
Aveo Pharmaceuticals, Inc.
International Classes:
C12N5/10; A01K67/027; C12N15/09; G01N33/15; G01N33/50
Foreign References:
| WO2002079419A1 |
Other References:
Sem. Cancer Biol.,1996年,vol. 7,299-306
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita