Title:
改変されたニトリラーゼおよびカルボン酸の製造方法におけるその使用
Document Type and Number:
Japanese Patent JP4584242
Kind Code:
B2
Abstract:
The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.
Inventors:
Tselinski, Thomas
Keseler, maria
Bernhard, Hauer
Friedrich, thomas
Keseler, maria
Bernhard, Hauer
Friedrich, thomas
Application Number:
JP2006501937A
Publication Date:
November 17, 2010
Filing Date:
February 24, 2004
Export Citation:
Assignee:
BASF SE
International Classes:
C12N9/78; A01H5/00; A01K67/027; C07K14/195; C07K14/21; C07K14/225; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N15/55; C12N15/82; C12P7/40
Foreign References:
| WO2001034786A1 | ||||
| WO2003000840A1 | ||||
| WO2000023577A1 |
Other References:
Biochim. Biophys. Acta,1998年,vol. 1382,1-4
GenBank Accession D82961,2002年 7月23日,URL,http://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?1167514:OLD04:4058513
Biochemistry,1990年,vol. 31,9000-9007
Proc. Natl. Acad. Sci. USA,1993年,vol. 90,247-251
GenBank Accession D82961,2002年 7月23日,URL,http://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?1167514:OLD04:4058513
Biochemistry,1990年,vol. 31,9000-9007
Proc. Natl. Acad. Sci. USA,1993年,vol. 90,247-251
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Eiichi Arai
Ishii Teiji
Satoshi Fujita
Eiichi Arai