Title:
萎縮型加齢黄斑変性の検査用キットおよび検査方法
Document Type and Number:
Japanese Patent JP6924179
Kind Code:
B2
Abstract:
The object of the present invention is to provide a method, using blood as a sample, for accurately and conveniently measuring Alu RNA, which is a diagnosis marker for atrophic age-related macular degeneration and a target of a therapeutic agent for atrophic age-related macular degeneration. According to the present invention, a method is provided for measuring an Alu RNA level in blood, comprising the following steps:
(1) carrying out a reverse transcription reaction on RNA extracted from a blood sample collected from a subject as a template, using a reverse transcription primer having a poly dT sequence and a universal tag sequence at the 5' side of the poly dT sequence, to synthesize cDNA, and
(2) carrying out PCR amplification on the cDNA synthesized in step (1) as a template, using a forward primer consisting of a nucleotide sequence comprising at least 15 contiguous nucleotides from a nucleotide sequence consisting of nucleotides at positions 1 to 117 in the nucleotide sequence as set forth in SEQ ID NO: 1, wherein the uracil of the nucleotides is replaced by the thymine, and a reverse primer consisting of a complementary sequence to the universal tag sequence or a partial sequence thereof, and measuring an Alu RNA level in blood based on an amount of the amplification product.
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Inventors:
Toshiyoshi Matsushita
Application Number:
JP2018507430A
Publication Date:
August 25, 2021
Filing Date:
March 24, 2017
Export Citation:
Assignee:
Santen Pharmaceutical Co., Ltd.
International Classes:
C12Q1/6883; C12Q1/686
Foreign References:
| US8809517 | ||||
| US20100112644 | ||||
| CN103966327A | ||||
| WO2010103522A1 |
Other References:
KANEKO H. et al.,NATURE,Vol.471 (2011),p.325-330
FOWLER B. et al,Acta Ophthalmologica,Vol.91 Issue s252 (2013),T081
TZU-HUEY Li et al.,Gene,276 (2001),p.135-141
***ALU WARNING: Human Alu-Sq subfamily consensus sequence.,Database GenBank [online], Accession No. U14573.1,1994年11月17日,[Retrieved on 2017.5.31],URL,
***ALU WARNING: Human Alu-J subfamily consensus sequence.,Database Genbank [online], Accession No.U14567.1,1994年11月17日,Retrieved on 2017.5.31,URL,
FOWLER B. et al,Acta Ophthalmologica,Vol.91 Issue s252 (2013),T081
TZU-HUEY Li et al.,Gene,276 (2001),p.135-141
***ALU WARNING: Human Alu-Sq subfamily consensus sequence.,Database GenBank [online], Accession No. U14573.1,1994年11月17日,[Retrieved on 2017.5.31],URL,
***ALU WARNING: Human Alu-J subfamily consensus sequence.,Database Genbank [online], Accession No.U14567.1,1994年11月17日,Retrieved on 2017.5.31,URL,
Attorney, Agent or Firm:
Hiraki International Patent Office