Title:
PRODUCTION OF SACCHARIDES CONTAINING SIALIC ACID
Document Type and Number:
Japanese Patent JP3124199
Kind Code:
B2
Abstract:
PURPOSE: To simply obtain saccharides containing sialic acid and facilitate the examination of physiological functions or biological meanings of saccharides containing the sialic acid by reacting cytidine monophosphate-sialic acid with an enzyme capable of transferring the sialic acid to a galactose residue, etc., in a conjugated carbohydrate saccharide chain or a free saccharide chain.
CONSTITUTION: Cytidine monophosphate-sialic acid is reacted with a β- galactoside α2,6-sialyltransferase derived from Photobacterium damsela JT0160, ATCC33539, ATCC35083, etc., to transfer the sialic acid to the 6-position of the galactose residue in a conjugated carbohydrate saccharide chain or a free saccharide chain or the 6-position of a monosaccharide capable of constituting the conjugated carbohydrate derived from an animal and having hydroxyl group at the 6-position. The transferase has 64000±5000 molecular weight (measured by the gel filtration), 5-6 optimum pH and 30°C optimum temperature.
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Inventors:
Gaku Yamamoto
Nakashizuka element
Ichiro Terada
Hisashi Kodama
Nakashizuka element
Ichiro Terada
Hisashi Kodama
Application Number:
JP32715294A
Publication Date:
January 15, 2001
Filing Date:
December 28, 1994
Export Citation:
Assignee:
Japan Tobacco Inc.
International Classes:
C12N9/10; C12P19/26; C12R1/01; (IPC1-7): C12P19/26
Attorney, Agent or Firm:
Yusuke Hiraki (1 person outside)