Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
LYMPHOMA GROWTH FACTOR
Document Type and Number:
Japanese Patent JPS58206528
Kind Code:
A
Abstract:

NEW MATERIAL:A substance having the following physical and chemical properties. Elementary analysis value (%): H 6.75, C 45.28, N 13.63. Specific rotatory power: [α]D30=-76.6. Solubility: easily soluble in water, 0.01N HCl and 0.01N NaOH. Color reaction: positive in biuret reaction, copper-Folin's reaction. Appearance: white powder, and its aqueous solution is colorless. Molecular weight: about 60 kilodalton.

USE: Having lymphoma growth effect, useful for preparing a physiologically active substance (e.g., interferon) by an animal cell (e.g., namarupa cell, or melanoma cell).

PROCESS: Grown bovine serum is gelled with glacial acetic acid, the gel is homogenized and centrifuged to give a supernatant liquid. The supernatant liquid is dialyzed to remove acetic acid, ultrafiltered, lyophilized, the dried material is suspended in a base medium for cultivating an animal cell, an active ingredient is extracted a soluble part is subjected to gel filtratin, and the active ingredient is purified to give the desired substance.


Inventors:
MURAKAMI HIRONORI
Application Number:
JP9034382A
Publication Date:
December 01, 1983
Filing Date:
May 27, 1982
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
MURAKAMI HIRONORI
International Classes:
A61K35/12; A61K35/14; A61K35/16; A61K35/26; A61K38/00; A61K38/16; A61K38/17; A61K38/18; C07K14/435; C07K14/52; C07K14/555; C12N5/00; C12N5/07; C12N5/071; (IPC1-7): A61K35/12; A61K35/16; A61K35/26; A61K37/00; A61K37/04; A61K37/24; C12N5/00
Attorney, Agent or Firm:
Shunji Nonami