MALTOSE-TREHALOSE CONVERSION ENZYME, ITS PRODUCTION AND USE THEREOF

Japanese Patent JP3633648

B2

PURPOSE: To obtain a new enzyme useful for converting maltose originated from starch into trehalose and a glucide containing trehalose in high conversion.
CONSTITUTION: This maltose.trehalose conversion enzyme has an action to convert maltose into trehalose and trehalose into maltose. It has a molecular weight of about 57,000-120,000 dalton (SDS-gel electrophoresis) and an isoelectric point pI of about 3.8-5.1 (ampholine-containing electrophoresis) and is inhibited by 1mM Cu2+, Hg2+ or 50 mM tris-HC1 buffer solution. The enzyme produced by a microbial strain of the genus Pimelobacter has the optimum temperature of about 20°C (pH 7.0, 60min) and the optimum pH of about 7.0-8.0 (25°C, 60min) and is stable at about ≤30°C (pH 7.0, 60min) and at pH 6.0-9.5 or thereabout (20°C, 60min). It can be produced by culturing Pimelobacter sp. R48 (FERM BP-4315) in a nutrient medium and collecting from the cultured product.

Tomoyuki Nishimoto
Hiroto Chaen
Toshiyuki Sugimoto
Toshio Miyake

JP14409294A

March 30, 2005

June 03, 1994

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Hayashibara Institute of Biochemistry

A23C9/13; A21D2/18; A23C9/18; A23G3/00; A23G3/02; A23G3/34; A23G3/42; A23G3/48; A23G4/00; A23G4/10; A23L2/39; A23L2/64; A23L11/20; A23L13/00; A23L13/40; A23L15/00; A23L27/30; A23L29/269; A61K8/00; A61K8/365; A61K8/60; A61K8/66; A61K8/73; A61K8/99; A61K9/06; A61K9/08; A61K9/20; A61K9/36; A61K31/70; A61K31/7016; A61Q11/00; A61Q17/04; A61Q19/00; A61Q19/02; C07H3/04; C12N1/20; C12N9/10; C12N9/90; C12P19/12; C12P19/14; C12P19/16; C12P19/20; C12P19/24; C13K13/00; A61Q7/00; C12R1/01; C12R1/40; (IPC1-7): C12N9/10; C12N1/20; C12P19/14

JP5211882A
JP58216695A

「『虫歯になりにくいオリゴ糖』添加菓子類の商品テスト結果 平成3年」,国民生活センター,平成3年1月発行
Trends in Food Science and Technology, 1991, Vol.2, No.7, p.166-169
日本結晶学会誌、1993年6月、第35巻、第3号、第220-222頁

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