Title:
MANUFACTURE OF MOUSE INTERLEUKIN 2
Document Type and Number:
Japanese Patent JPS57175121
Kind Code:
A
Abstract:
A process for preparing murine IL-2 from malignant neoplastic cells includes culturing murine leukemia or lymphoma cells in vitro in a protein containing medium supplemented with various additives. An optimum concentration of a T cell mitogen is added to the culture medium to stimulate maximum production of a supernate which contains IL-2. After a period of time, the supernate is collected and purified into more concentrated form. Phorbol myristate acetate may be added to a suboptimum concentration of the T cell mitogen to reduce the quantity of the mitogen required to produce maximum quantities of murine IL-2.
Inventors:
SUTEIIBUN GIRISU
Application Number:
JP7639481A
Publication Date:
October 28, 1982
Filing Date:
May 20, 1981
Export Citation:
Assignee:
SUTEIIBUN GIRISU
International Classes:
C12P21/00; A61K35/26; A61K38/16; C07K14/435; C07K14/52; C07K14/54; C07K14/55; (IPC1-7): A61K35/12; A61K35/14; A61K35/26; A61K37/02; C07G7/00; C12P21/00
Other References:
THE JOURNAL OF IMMUNOLOGY=1980
BEHRING INSTITUTE MITTEILUNGEN=1980
BEHRING INSTITUTE MITTEILUNGEN=1980
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