Title:
MANUFACTURE OF STROMA-FREE, HEPATITIS-FREE AND HIGH PURITY HUMAN AND ANIMAL HEMOGLOBIN SOLUTION
Document Type and Number:
Japanese Patent JPS60224626
Kind Code:
A
Abstract:
To provide a method that allows the preparation, not only from human blood but also from animal blood and in quantities large enough for clinical applications, of highly purified hemoglobin solutions that can be employed for the treatment of humans and that are free of plasma proteins and residual stroma lipids (a) a starting material that contains erythrocytes is pumped over a filter unit that has a pore size between 0.1 and 1.2 mu m in circulation against an isotonic washing solution, (b) the product of (a) is hemolyzed and pumped over an ultrafiltration unit that has a permeability of 80,000 to 100,000 D in circulation against water, and (c) the ultrafiltrate obtained in stage (b) is pumped and diafiltered over an ultrafiltration unit that has a permeability of 10,000 to 50,000 D in circulation against water, whereby concentration is conducted before and/or after diafiltration.
Inventors:
DOKUTAA NORUBERUTO KOTE
DOKUTAA BERUTORAMU IICHIENTOFU
DOKUTAA BERUTORAMU IICHIENTOFU
Application Number:
JP6672885A
Publication Date:
November 09, 1985
Filing Date:
April 01, 1985
Export Citation:
Assignee:
BIOTEST PHARMA GMBH
International Classes:
A61K35/14; A61K35/18; A61K38/16; C07K14/00; C07K14/805; A61K38/00; (IPC1-7): A61K35/18; A61K37/14
Attorney, Agent or Firm:
Kinichi Kitamura