To provide a manufacturing method of a display substrate that can easily remove un unnecessary adhered substance adhered to the top of partition walls with high precision.

On a substrate 1, partition walls 3 for dividing a surface of the substrate into a plurality of regions are formed, and a color filter layer 4 and a light emitting layer 5 are formed in the regions. Then, a water solvable polymer layer 6 is coated at the top of the partition walls 3, and a planarized layer 7 is formed on the light emitting layer 5. Then, the water solvable polymer layer 6 formed on the top of the partition walls 3 is removed by water rinsing to remove un unnecessary adhered substance adhered to the top of the partition walls 3.