Title:
MANUFACTURING METHOD OF REFINED PRODUCTS OF EPIDERMAL GROWTH FACTOR THAT IS EXPRESSED WITHIN PLANT BODY
Document Type and Number:
Japanese Patent JP2016172707
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide means of increasing the yield of the refined products of EGF protein that is expressed within a plant body.SOLUTION: The invention provides a manufacturing method of the refined products of the epidermal growth factor that is expressed within a plant body comprising: a crushing step of crushing a plant body containing epidermal growth factor; a centrifugation step of centrifuging a crushing solution and recovering a supernatant; a two-phase partition step of subjecting the recovered supernatant to the aqueous two-phase partition method using the aqueous two-phase system consisting of a polyethyleneglycol phase and a salt phase, and recovering a polyethyleneglycol phase; and a chromatography step of subjecting the polyethyleneglycol phase to an ion exchange chromatography or adsorption chromatography; and comprising no ultrafiltration step.SELECTED DRAWING: Figure 1
Inventors:
YOSHINAKA KYOJI
SATO SEIYA
OKAWA SHOICHIRO
SATO SEIYA
OKAWA SHOICHIRO
Application Number:
JP2015054286A
Publication Date:
September 29, 2016
Filing Date:
March 18, 2015
Export Citation:
Assignee:
UNIBIO CORP
International Classes:
C07K14/475; A61K8/64; A61K38/22; A61P17/02; A61P43/00; A61Q19/00; C07K1/36
Domestic Patent References:
| JP2013516460A | 2013-05-13 | |||
| JPH09501931A | 1997-02-25 | |||
| JP2012530131A | 2012-11-29 |
Foreign References:
| WO2014097733A1 | 2014-06-26 |
Other References:
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GU, ZHENGRONG: "Recovery of recombinant proteins from plants using aqueous two-phase partitioning systems: An outli", METHODS IN MOLECULAR BIOLOGY, vol. Vol.1129, JPN6018050665, 2014, pages 77 - 87
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JOHANNES F. BUYEL ET AL.: "Flocculation increases the efficacy of depth filtration during the downstream processing of recombin", PLANT BIOTECHNOL. J., vol. vol.12, JPN6018050667, 2014, pages 240 - 252
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PLATIS DIMITRIS: "Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from", J. CHROMATOGR. A, vol. Vol.1128, No.1-2, JPN6018050670, 2006, pages 114 - 124
GU, ZHENGRONG: "Recovery of recombinant proteins from plants using aqueous two-phase partitioning systems: An outli", METHODS IN MOLECULAR BIOLOGY, vol. Vol.1129, JPN6018050665, 2014, pages 77 - 87
AGUILAR, OSCAR ET AL.: "Aqueous two-phase systems strategies for the recovery and characterization of biological products fr", J. SCI. FOOD AGRIC., vol. 90, no. 9, JPN6018050666, 2010, pages 1385 - 1392, XP055504927, DOI: doi:10.1002/jsfa.3956
JOHANNES F. BUYEL ET AL.: "Flocculation increases the efficacy of depth filtration during the downstream processing of recombin", PLANT BIOTECHNOL. J., vol. vol.12, JPN6018050667, 2014, pages 240 - 252
JOHANNES FELIX BUYEL ET AL.: "Downstream processing of biopharmaceutical proteins produced in plants The pros and cons of floccul", BIOENGINEERED, vol. 5, no. 2, JPN6018050668, 2014, pages 138 - 142
LEE, JAE-HWA: "Partitioning of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) from pl", BIOTECHNOL. BIOPROCESS ENG., vol. 9, no. 1, JPN6018050669, 2004, pages 12 - 16
PLATIS DIMITRIS: "Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from", J. CHROMATOGR. A, vol. Vol.1128, No.1-2, JPN6018050670, 2006, pages 114 - 124
Attorney, Agent or Firm:
Patent Service Corporation Tanigawa International Patent Office