PURPOSE: To qualitatively or quantitatively measure an in vivo material simply at high sensitivity by reacting an antigen in the in vivo material with an antibody of mixed particles carried with the antibody or antigen on two kinds of magnetic particles having the ratios between magnetization quantities and grain sizes within the specific ranges and particular specific weights.
CONSTITUTION: The antibody of mixed particles and an antigen in an in vivo material, or the antigen of the mixed particles and an antibody in the in vivo material are reacted with the mixed particles carried with the antibody or antigen on the magnetic particles having the ratio between the magnetization quantity and grain size within the ranges of A, B, C, D, E and the specific weight of 1-2 and the magnetic particles having the ratio between the magnetization quantity and grain size within the ranges of C', D', E', F, G and the specific weight of 1-2. The magnetic field is applied to the reaction mixture. The absorbance or transmittance is measured with the wavelength of 350nm or above on unmagnetized magnetic particles, and the antigen or antibody in the in vivo material is qualitatively or quantitatively measured. Cross-linked polymerization particles, organic polymer material, or inorganic fine powder is used for the magnetic particles.
KOBAYASHI FUMIKA
NAGAHATA SATOSHI
ANAMI MAKOTO
