Title:
一本鎖抗体によるサイトゾルタンパク質の機能阻害法およびその利用
Document Type and Number:
Japanese Patent JP4938263
Kind Code:
B2
Abstract:
The invention relates to a method of inhibiting a function of a cytosolic protein by a single chain antibody (scFv). More specifically, it relates to a method of inhibiting the function of a cytosolic protein by using a single chain antibody constructed by removing a signal peptide of one of VH and VL of an anti-cytosolic protein antibody, a method of analyzing the function of a cytosolic protein by using the single chain antibody, a method of producing the single chain antibody which has a characteristic of specifically inhibiting the function of a cytosolic protein and a non-human transgenic animal in which the single chain antibody which has a characteristic of specifically inhibiting the function of a cytosolic protein is expressed and the function of a cytosolic protein is inhibited. It was revealed that a scFv gene (SHL) in which VH and VL of an anti-WASP-EVH1 monoclonal antibody gene were utilized and VH having a leader sequence and VL in which a leader sequence was removed were connected with a linker and a gene (SHL-CL) having a CL sequence were expressed in the cytosol and inhibited the function of a cytosolic protein.
Inventors:
Kenji Sekikawa
Mitsuru Sato
Yoshikazu Kurosawa
Mitsuru Sato
Yoshikazu Kurosawa
Application Number:
JP2005238132A
Publication Date:
May 23, 2012
Filing Date:
August 19, 2005
Export Citation:
Assignee:
NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
International Classes:
C07K16/18; A01K67/027; C07K16/46; C12N5/10; C12N15/09; C12P21/08; C12Q1/02; G01N33/53; A61K39/395; A61P43/00
Other References:
日本分子生物学会年会プログラム・講演要旨集, 2003, vol.26th, p.910(3PC-127)
臨床免疫, 2001, vol.36, no.2, p.238-244
Nat Rev Mol Cell Biol., 2002, vol.3, no.4, p.246-255
EMBO J., 2003, vol.22, no.10, p.2309-2317
Nature, 2004, vol.429, no.6994, p.841-847
Gene, 187(1997) p.1-8
臨床免疫, 2001, vol.36, no.2, p.238-244
Nat Rev Mol Cell Biol., 2002, vol.3, no.4, p.246-255
EMBO J., 2003, vol.22, no.10, p.2309-2317
Nature, 2004, vol.429, no.6994, p.841-847
Gene, 187(1997) p.1-8
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi