Title:
トランスジェニック植物における多価不飽和脂肪酸の製造方法
Document Type and Number:
Japanese Patent JP4567047
Kind Code:
B2
Abstract:
Producing polyunsaturated fatty acids (I) comprises introducing nucleic acid sequences coding for delta 9 elongase or delta 6 desaturase, delta 8 desaturase or delta 6 elongase, delta 5 desaturase, delta 5 elongase and delta 4 desaturase activity into an organism to produce a transgenic organism whose total lipids include at least 1 wt.% (I). Producing polyunsaturated fatty acids of formula (I) comprises introducing nucleic acid sequences coding for delta 9 elongase or delta 6 desaturase, delta 8 desaturase or delta 6 elongase, delta 5 desaturase, delta 5 elongase and delta 4 desaturase activity into an organism to produce a transgenic organism whose total lipids include at least 1 wt.% of formula (I). [Image] R 1>OH, coenzyme A (thioester), lysophosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl glycerol, lysophosphatidyl serine, lysophosphatidyl inositol, sphingobase or OCH 2CH(OR 3>)CH 2OR 2>; R 2>H, lysophosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl glycerol, lysophosphatidyl serine, lysophosphatidyl inositol, (un)saturated 2-24C alkylcarbonyl or CO(CH 2) n(CH=CHCH 2) m(CH 2) pCH 3; R 3>H, (un)saturated 2-24C alkylcarbonyl or CO(CH 2) n(CH=CHCH 2) m(CH 2) pCH 3; n : 2-9; m : 2-6; p : 0 or 3. Independent claims are also included for: (1) oils, lipids or fatty acids produced as above; (2) oil, lipid or fatty acid composition that comprises polyunsaturated fatty acids produced as above and is derived from transgenic plants; (3) isolated nucleic acid sequence coding for a delta 5 elongase polypeptide having one of 6 amino acid sequences given in the specification; (4) isolated nucleic acid sequence that codes for a delta 6 elongase polypeptide and has one of 4 nucleotide sequences given in the specification; (5) isolated nucleic acid sequence that codes for a omega 3 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (6) isolated nucleic acid sequence that codes for a delta 6 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (7) isolated nucleic acid sequence that codes for a delta 5 desaturase polypeptide and has one of 4 nucleotide sequences given in the specification; (8) isolated nucleic acid sequence that codes for a delta 4 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (9) isolated nucleic acid sequence that codes for a delta 12 desaturase polypeptide and has one of 2 nucleotide sequences given in the specification; (10) amino acid sequence encoded by an isolated nucleic acid sequence as above; (11) genetic construct comprising an isolated nucleic acid as above linked to one or more regulatory signals; (12) vector containing a nucleic acid or construct as above; (13) transgenic non-human organism containing a nucleic acid, construct or vector as above.
Inventors:
Syrups, Petra
Jörg, Bauer
Kiu, Xiao
Wu, Guohai
Datra, Nagamani
Jörg, Bauer
Kiu, Xiao
Wu, Guohai
Datra, Nagamani
Application Number:
JP2007500134A
Publication Date:
October 20, 2010
Filing Date:
February 23, 2005
Export Citation:
Assignee:
BSF Plant Science Game Beher
International Classes:
C12N15/09; A01H1/00; A01H5/00; C12P7/64; C12P9/00; A23D9/00; A61K8/36; A61K8/37; A61K8/92; A61K31/202; A61K31/232; A61K36/18; A61P3/02; A61P3/04; A61P3/06; A61P3/10; A61P9/00; A61P9/12; A61P19/02; A61P25/00; A61P27/02; A61P29/00; A61P35/00; C11B11/00; C11C3/00
Foreign References:
| WO2003064638A1 | ||||
| WO2003074715A1 | ||||
| WO2000075341A1 |
Other References:
Proc.Natl.Acad.Sci.USA, 97[12](2000) p.6421-6426
J.Plant Physiol., 160(2003) p.779-802
THE PLANT CELL, 16(Oct 2004) p.2734-2748
Biochimie, 86(available online 19th Oct 2004) p.793-798
J.Plant Physiol., 160(2003) p.779-802
THE PLANT CELL, 16(Oct 2004) p.2734-2748
Biochimie, 86(available online 19th Oct 2004) p.793-798
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Ishii Teiji
Satoshi Fujita