Title:
METHOD FOR ACQUIRING GENE TAG
Document Type and Number:
Japanese Patent JP3845416
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for acquiring a gene tag and a method for analyzing the gene tag.
SOLUTION: A method for generating 5' terminal base sequence of an mRNA as a tag is provided. The method of the invention includes a process for synthesizing cDNA using mRNA connecting a IIs linker containing the recognition sequence of a IIs type restriction enzyme as a template to CAP (catabolite gene activator protein) structure. The tag comprising 5' terminal base sequence is generated by acting the IIs restriction enzyme to the cDNA. The tag is generated from every mRNA without depending on the base sequence. A method for identifying the initial point of transcription and a primer for synthesizing the total length cDNA are provided depending on the information on the base sequence of the tag.
Inventors:
Shinichi Hashimoto
Koji Matsushima
Sumio Sugano
Koji Matsushima
Sumio Sugano
Application Number:
JP2004006630A
Publication Date:
November 15, 2006
Filing Date:
January 14, 2004
Export Citation:
Assignee:
Post Genome Institute Co., Ltd.
International Classes:
C12N15/09; C07K14/00; C07K14/47; C07K16/00; C07K16/18; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/10; C12N15/12; C12P21/02; C12Q1/68; (IPC1-7): C12N15/09; C07K14/00; C07K16/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02; C12Q1/68
Other References:
BioTechniques, 2001, Vol.30, No.4, p.892-897
実験医学, 1999, 別冊, p.285-292
実験医学, 1999, 別冊, p.285-292
Attorney, Agent or Firm:
Hatsushi Shimizu