Title:
METHOD FOR ACTIVATING DENATURED PROTEIN
Document Type and Number:
Japanese Patent JP3791328
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for efficiently activating a denatured protein and a kit for protein refolding.
SOLUTION: This method for activating a denatured protein comprises a process for adding a surfactant to a denatured protein to form a protein/ surfactant complex and a process for adding a high-molecular weight amylose to the protein/surfactant complex to remove the surfactant by the high-molecular weight amylose. This kit for protein refolding is provided with at least one kind of a high-molecular weight amylose and at least one kind of a surfactant.
Inventors:
Sachiko Machida
Lin Qing
Takaba Takeshi
Yoshinobu Terada
Kazutoshi Fujii
Lin Qing
Takaba Takeshi
Yoshinobu Terada
Kazutoshi Fujii
Application Number:
JP2000355064A
Publication Date:
June 28, 2006
Filing Date:
November 21, 2000
Export Citation:
Assignee:
Incorporated administrative agency Food Research Institute
Ezaki Glico Co., Ltd.
Ezaki Glico Co., Ltd.
International Classes:
C07K1/08; C12N9/04; C07K1/113; C12N9/36; C12N9/88; C12N9/00; (IPC1-7): C07K1/08; //C12N9/04; C12N9/36; C12N9/88
Domestic Patent References:
| JP2001261697A |
Other References:
SUNDARI C. Sivakama et al.,Artificial chaperoning of insulin,human carbonic anhydrase and hen egg lysozyme using linear dextrin,FEBS Letters,1999年,443,215-219
DAUGHERTY David L. et al.,Artificial Chaperone-assisted Refolding of Citrate Synthase,THE JOURNAL OF BIOLOGICAL CHEMISTRY,1998年,273(51),33961-33971
DAUGHERTY David L. et al.,Artificial Chaperone-assisted Refolding of Citrate Synthase,THE JOURNAL OF BIOLOGICAL CHEMISTRY,1998年,273(51),33961-33971
Attorney, Agent or Firm:
Hidesaku Yamamoto
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