To provide a method and an apparatus for carrying out hybridization by which a specific binding substance is efficiently hybridized with a substance derived from an organism and data for biochemical analysis having good reproducibility and excellent in quantitative property can be prepared.

This hybridization apparatus is equipped with a reacting vessel 11 in which a plurality of absorbing areas containing the specific binding substance having well-known structure or characteristic are formed mutually separately through an opening 11a of the upper end and a unit holder for biochemical analysis in which the vicinity of the one end of the holder 7 is nipped by a nipping member is made chargeable and a hybridization solution-injecting pin, a probe solution-injecting pin capable of injecting a probe solution containing the substance derived from the organism labeled by a labeling substance a washing solution-injecting pin, a solution-pulling out pin for pulling out the solution stored in the reacting vessel, a nipping member capable of nipping the unit holder for biochemical analysis and a driving motor for vertically moving the nipping member.