Title:
METHOD FOR CREATING PROBE DNA TO NUCLEIC ACID ENCODING MEMBRANE PROTEIN, METHOD FOR DETECTING EXPRESSION OF GENE ENCODING MEMBRANE PROTEIN, AND PROBE DNA-IMMOBILIZING CARRIER
Document Type and Number:
Japanese Patent JP2005224189
Kind Code:
A
Abstract:
To provide a method for rapidly and comprehensively detecting expression of a gene encoding a membrane protein.
The method for creating a probe DNA to a nucleic acid encoding the membrane protein comprises selecting a partial region of a gene which encodes the membrane protein, in which ≥5% of the base sequence comprises an exon sequence encoding a region essential to the membrane binding in the membrane protein, and which contains cytosine and guanine in 40-60% proportion as the total, and creating a DNA comprising the base sequence of the partial region, or a base sequence complementary to the partial region.
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Inventors:
SENOO SHOJI
TAKUA
OKAMURA HIROSHI
HIRAYAMA KOICHI
TAKUA
OKAMURA HIROSHI
HIRAYAMA KOICHI
Application Number:
JP2004037041A
Publication Date:
August 25, 2005
Filing Date:
February 13, 2004
Export Citation:
Assignee:
TOYO KOHAN CO LTD
SENOO SHOJI
SENOO SHOJI
International Classes:
G01N33/53; C12M1/00; C12N15/09; C12Q1/00; C12Q1/68; G01N33/551; G01N33/566; (IPC1-7): C12N15/09; C12M1/00; C12Q1/68; G01N33/53; G01N33/551; G01N33/566
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Naomi Shimamura
Ishii Teiji
Satoshi Fujita
Naomi Shimamura