Title:
酵素を用いる電極マイクロアレイにおける結合事象の検出方法
Document Type and Number:
Japanese Patent JP2009510485
Kind Code:
A
Abstract:
The present invention provides a process to detect binding events on an electrode microarray. A microarray is provided having addressable electrodes and two or more different types of capture complexes at sites corresponding to the electrodes. The capture complexes capture analytes. Enzymes are attached to form a reporter complex. Substrate solutions are sequentially contacted to make enzyme products that are detectable at the electrodes by a difference in the electrical response at electrodes having the enzyme product and those not having the enzyme product. The enzyme product may be a solid deposition product. The electrical properties of electrodes on the microarray are read for the presence of the enzyme product by sequentially switching each electrode held at a constant voltage to ground and then back to the constant voltage.
Inventors:
Cillian Dill
John Jay Cooper Jr.
Andrey Gindylis
Christian M Ross
John Jay Cooper Jr.
Andrey Gindylis
Christian M Ross
Application Number:
JP2008541161A
Publication Date:
March 12, 2009
Filing Date:
September 20, 2006
Export Citation:
Assignee:
CombiMatrix Corporation
International Classes:
G01N27/416; C12Q1/26; C12Q1/34; C12Q1/42; C12Q1/68; G01N27/00; G01N27/327; G01N33/53; G01N33/543; G01N37/00
Domestic Patent References:
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Attorney, Agent or Firm:
Kyousei Tamura
Samejima Mutsumi
Masahiro Nitta
Samejima Mutsumi
Masahiro Nitta