ジンクフィンガー蛋白質を用いる標的核酸の検出方法 - Tokyo University of Agriculture and Technology TLO Co., Ltd.

Title:

ジンクフィンガー蛋白質を用いる標的核酸の検出方法

Document Type and Number:

Japanese Patent JP4336814

Kind Code:

B2

Abstract:

To provide a method for specifically detecting a target nucleic acid through developing an assaying system enabling a PCR (polymerase chain reaction) product to be specifically detected as it is in a double-stranded form.

This method for detecting the target nucleic acid in a specimen comprises the step(a) of amplifying the nucleic acid in the specimen using a pair of primers by a PCR reaction, wherein the pair of primers is so designed that a zinc finger protein recognition sequence present in the target nucleic acid is amplified by the PCR reaction, and the step(b) of detecting the PCR amplified product obtained in the step(a) using the zinc finger protein. As the zinc finger protein, Zif268 which recognizes, e.g. sequence 5'-GCGTGGGCG-3' can be used.

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Inventors:

Kazunori Ikebukuro

Application Number:

JP2003285706A

Publication Date:

September 30, 2009

Filing Date:

August 04, 2003

Export Citation:

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Assignee:

Tokyo University of Agriculture and Technology TLO Co., Ltd.

International Classes:

C12Q1/68; G01N33/53; C12N15/09; G01N33/566; G01N37/00

Domestic Patent References:

JP2002125700A
JP10504517A
JP2002139491A

Foreign References:

WO2002008286A1

Other References:

Proc. Natl. Acad. Sci. USA,2001年,Vol.98, No.13,pp.7158-7163
生物物理化学,2001年,第45巻,第4号,pp.235-240
J. Clin. Microbiol.,2002年,Vol.40, No.8,pp.2779-2785

Attorney, Agent or Firm:

Seiji Ohno
Koji Morita
Reiko Tanaka

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