METHOD FOR DISCRIMINATING α2,6-SIALO-SUGAR CHAIN FROM α2,3-SIALO-SUGAR CHAIN

Japanese Patent JP2013076629

A

To provide a method for allowing efficient discrimination of α2,6-sialo-sugar chain included in a biological sample from α2,3-sialo-sugar chain.

Methyl esterification using a 1-methyl-3-p-tolyltriazene (MTT) reagent is applied to a sialo-sugar chain in a sugar chain sample captured in a carrier, and then a sugar chain is isolated. A methyl-esterified α2,3-sialo-sugar chain in the isolated sugar chain is demethylated under an acid condition; reductive amination reaction is performed by reacting the isolated sugar chain with a compound having an amino group to label the sugar chain by the compound having the amino group. By subjecting thus obtained labeled sugar chain to high-speed liquid chromatography or mass analysis, the α2,6-sialo-sugar chain and the α2,3-sialo-sugar chain can be efficiently discriminated from each other.

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TOYODA MASATETSU

JP2011216474A

April 25, 2013

September 30, 2011

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SUMITOMO BAKELITE CO

G01N27/62; G01N30/00; G01N30/04; G01N30/06; G01N30/72; G01N30/88

JP2009216609A	2009-09-24
JP2009229426A	2009-10-08
JP2001029095A	2001-02-06

WO2007099856A1	2007-09-07
WO2011038873A1	2011-04-07

JPN6015017073; 富岡　あづさ，　亀山　昭彦: '「ＭＡＬＤＩ-ＱＩＴ-ＴＯＦ　ＭＳによるメチルエステル化シアロ糖鎖の解析」' 第５６回質量分析総合討論会講演要旨集 , 20080501, ｐ．　４８２-４８３, 日本質量分析学会
JPN6015017073; 富岡　あづさ，　亀山　昭彦: '「ＭＡＬＤＩ-ＱＩＴ-ＴＯＦ　ＭＳによるメチルエステル化シアロ糖鎖の解析」' 第５６回質量分析総合討論会講演要旨集 , 20080501, ｐ．　４８２-４８３, 日本質量分析学会

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