METHOD FOR EFFICIENTLY TRANSFECTING CELL WITH POLYNUCLEOTIDE USING TRANSFECTING REAGENT

Title:

METHOD FOR EFFICIENTLY TRANSFECTING CELL WITH POLYNUCLEOTIDE USING TRANSFECTING REAGENT

Document Type and Number:

Japanese Patent JP2004113099

Kind Code:

A

Abstract:

To provide a method for transfecting a cell with a polynucleotide for further improving the gene transfer efficiency in a versatile electroporation process to acquire and express gene clones having been unable to be detected for the reason of low abundance, or the like.

This method is characterized in including carrying out the electroporation process in the presence of the transfecting reagent.

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Inventors:

KAWADA ETSUKAZU
YANO SHINICHI
KOJIMA HIROYUKI

Application Number:

JP2002280325A

Publication Date:

April 15, 2004

Filing Date:

September 26, 2002

Export Citation:

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Assignee:

NAT INST OF ADV IND & TECHNOL

International Classes:

C12N15/09; (IPC1-7): C12N15/09

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