Title:
がん特異的突然変異に対し抗原特異性を有するＴ細胞受容体を単離する方法
Document Type and Number:
Japanese Patent JP7437444
Kind Code:
B2
Abstract:
To provide methods for isolating T cell receptors having antigen specificity to cancer specific mutations.SOLUTION: Disclosed is a method for isolating a TCR having an antigen specificity to a mutated amino acid sequence encoded by a cancer-specific mutation, the method comprising the steps of: identifying one or more genes each having a cancer specific mutation encoding a mutated amino acid sequence in the nucleic acid of a cancer cell of a patient; inducing autologous APCs of the patient to present the mutated amino acid sequence; co-culturing the autologous T cells with the autologous APC presenting the mutated amino acid sequence; selecting autologous T cells; and isolating a nucleotide sequence encoding the TCR from the selected autologous T cell, where the TCR has antigen specificity to the mutated amino acid sequence encoded by the cancer specific mutation.SELECTED DRAWING: None
More Like This:
Inventors:
Tran, Eric
Le, Yong-Cheng
Robins, Paul F.
Rosenberg, Stephen A.
Le, Yong-Cheng
Robins, Paul F.
Rosenberg, Stephen A.
Application Number:
JP2022077983A
Publication Date:
February 22, 2024
Filing Date:
May 11, 2022
Export Citation:
Assignee:
THE UNITED STATES OF AMERICA
International Classes:
A61K35/17; A61K9/08; A61K38/17; A61K39/00; A61P35/00; C07K14/705; C12N5/0781; C12N5/0783; C12N5/0784; C12N5/0786
Domestic Patent References:
| JP2012213402A | ||||
| JP2014023445A | ||||
| JP2013507986A |
Foreign References:
| WO2014043441A1 | ||||
| WO2014133567A1 | ||||
| WO2013039889A1 | ||||
| WO2014133568A1 |
Other References:
Nature Reviews Immunology (2012) Vol.12, pp.269-281
ROBBINS P.,Use of high throughput sequencing methods to identify cancer immunotherapy targets,日本免疫学会総会・学術集会記録 (2013) Vol.42, p.12, S7-2
Journal of Virology (1997) Vol.71, No.3, pp.2292-2302
Nature Medicine (2013) Vol.19, No.6, pp.747-752
Nature Medicine (2013) Vol.19, No.11, pp.1542-1546, Supplementary Figures and Tables
BMC Bioinformatics (2010) Vol.11, No.568, pp.1-12
The Journal of Immunology (2013) Vol.190, pp.6034-6042
ROBBINS P.,Use of high throughput sequencing methods to identify cancer immunotherapy targets,日本免疫学会総会・学術集会記録 (2013) Vol.42, p.12, S7-2
Journal of Virology (1997) Vol.71, No.3, pp.2292-2302
Nature Medicine (2013) Vol.19, No.6, pp.747-752
Nature Medicine (2013) Vol.19, No.11, pp.1542-1546, Supplementary Figures and Tables
BMC Bioinformatics (2010) Vol.11, No.568, pp.1-12
The Journal of Immunology (2013) Vol.190, pp.6034-6042
Attorney, Agent or Firm:
Hajime Takashima
Mitsuyoshi Kamada
Kyoko Doi
Yaeko Tamura
Hirofumi Taima
Atsuko Akai
Tomiya Tosaki
Masamichi Naka
Keijiro Kamei
Mitsuyoshi Kamada
Kyoko Doi
Yaeko Tamura
Hirofumi Taima
Atsuko Akai
Tomiya Tosaki
Masamichi Naka
Keijiro Kamei