To determine endotoxin in phospholipid accurately by emulsifying or dispersing phospholipid into water containing an anionic polymer metal salt and an alkaline metal salt, removing phospholipid from the water and then determining endotoxin in the remaining water.
0.01-0.5 pts.wt. of anionic polymer metal salt, e.g. sodium polyacrylate, having weight-average molecular weight of 500-30000, and 0.01-2 pts.wt. of alkaline metal salt, e.g. lithium fluoride, are added to 100 pts.wt. of sodium chloride brine. 0.1-5 pts.wt., preferably 0.5-2 pts.wt., of phospholipid derived from a natural product or synthesized phospholipid or liposome formed therefrom is then added to 100 pts.wt. of the aqueous solution and the phospholipid is emulsified or dispersed into the aqueous solution by means of a stirrer or an emulsifier. Subsequently, the phospholipid is separated and removed by filtering or centrifugal separation and the remaining aqueous solution is subjected to colorimetry thus determining endotoxin.
KISHIMOTO YOKO
SAITO KOICHI
TOKUYAMA SATORU
