PURPOSE: To obtain efficiently riboflavin, by cultivating a microorganism capable of producing the riboflavin using a lower aliphatic compound as a substrate, extracting the resultant culture with hot water, and cooling the resultant hot aqueous solution to crystallize the riboflavin.
CONSTITUTION: A microorganism capable producing riboflavin is cultivated using 1W4C lower aliphatic compound, e.g. acetic acid, methanol, ethanol, butanol or glycerol, as a substrate. Thus, the microorganism capable of producing the riboflavin, e.g. Saccharomyces cerevisiae P-154 (FERM-P No. 7562), is cultivated in a fermentation culture medium [containing about 103g/l calcium acetate, about 3g/l (NH4)2SO4, about 2g/l KH2PO4, about 1g/l MgSO4.7H2O, about 2.2mg/l ZnSO4.7H2O and about 1g/l adenine, about 7pH], and the resultant precipitate consisting of the yeast cells and riboflavin crystals is extracted with hot water. The resultant extract solution is then cooled to obtain the aimed riboflavin crystals.
YOSHIKANE MASAYOSHI
MATSUYAMA AKIKAZU
TAKAO SHIYOUICHI
| JPS5627239A | 1981-03-17 | |||
| JPS5119187A | 1976-02-16 | |||
| JPS5610034A | 1981-02-02 | |||
| JPS50116690A | 1975-09-12 | |||
| JP28002646A |