Title:
ｉＰＳ細胞からの血小板の調製方法
Document Type and Number:
Japanese Patent JP5617631
Kind Code:
B2
Abstract:
It is an object of the present invention to provide a method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells in an in vitro culture system. The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, which is obtained by inoculating iPS cells onto feeder cells and then culturing the iPS cells under conditions suitable for inducing the differentiation of hematopoietic progenitor cells. Moreover, the present invention also provides a method for producing various types of blood cells, which comprises culturing hematopoietic progenitor cells enclosed in the sac-like structure under conditions suitable for inducing the differentiation of blood cells. Furthermore, the present invention also provides a method for producing various types of blood cells, particularly megakaryocytes and platelets, without involving the sac-like structure.
Inventors:
中内 啓光
江藤 浩之
錦井 秀和
高山 直也
山中 伸弥
高橋 和利
江藤 浩之
錦井 秀和
高山 直也
山中 伸弥
高橋 和利
Application Number:
JP2010505402A
Publication Date:
November 05, 2014
Filing Date:
April 01, 2009
Export Citation:
Assignee:
国立大学法人東京大学
International Classes:
C12N5/078; A61K35/12
Domestic Patent References:
| JP2007089432A | 2007-04-12 | |||
| JP2005287479A | 2005-10-20 |
Foreign References:
| WO2008041370A1 | 2008-04-10 | |||
| US20060099198A1 | 2006-05-11 | |||
| WO2007069666A1 | 2007-06-21 | |||
| WO2008041370A1 | 2008-04-10 |
Other References:
JPN6007014303; 高山直也ら: 'ヒトＥＳ細胞由来造血支持構造体：嚢状構造物（ＥＳ-Ｓａｃ）からの血液細胞の分化誘導' 臨床血液 ４８（９）, 2007, ｐ．９３６
JPN6009021396; ＴＡＫＡＹＡＭＡ， Ｎ．， ｅｔ ａｌ．: 'Ｈｕｍａｎ Ｅｍｂｒｙｏｎｉｃ Ｓｔｅｍ Ｃｅｌｌ-Ｄｅｒｉｖｅｄ "ＮＥＴ-Ｌｉｋｅ" Ｓｔｒｕｃｔｕｒｅ Ｓｅｒｖｅｓ ａｓ ａ Ｈｅｍａｔｏｐｏｉｅｔｉｃ Ｐｒｏｇｅｎｉｔｏｒ Ｎｉｃｈｅ ａｎ' Ｂｌｏｏｄ １０８（１１）（ｐａｒｔ １）, 20061116, ｐ．４７４ａ （要旨番号１６６５）
JPN6013062093; ＦＵＪＩＭＯＴＯ， Ｔ．-Ｔ．， ｅｔ ａｌ．: Ｂｌｏｏｄ １０２（１２）, 2003, ｐｐ．４０４４-４０５１
JPN6009021398; ＹＵ， Ｊ．， ｅｔ ａｌ．: 'Ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌ ｌｉｎｅｓ ｄｅｒｉｖｅｄ ｆｒｏｍ ｈｕｍａｎ ｓｏｍａｔｉｃ ｃｅｌｌｓ．' Ｓｃｉｅｎｃｅ ３１８, 2007, ｐｐ．１９１７-１９２０
JPN6009021396; ＴＡＫＡＹＡＭＡ， Ｎ．， ｅｔ ａｌ．: 'Ｈｕｍａｎ Ｅｍｂｒｙｏｎｉｃ Ｓｔｅｍ Ｃｅｌｌ-Ｄｅｒｉｖｅｄ "ＮＥＴ-Ｌｉｋｅ" Ｓｔｒｕｃｔｕｒｅ Ｓｅｒｖｅｓ ａｓ ａ Ｈｅｍａｔｏｐｏｉｅｔｉｃ Ｐｒｏｇｅｎｉｔｏｒ Ｎｉｃｈｅ ａｎ' Ｂｌｏｏｄ １０８（１１）（ｐａｒｔ １）, 20061116, ｐ．４７４ａ （要旨番号１６６５）
JPN6013062093; ＦＵＪＩＭＯＴＯ， Ｔ．-Ｔ．， ｅｔ ａｌ．: Ｂｌｏｏｄ １０２（１２）, 2003, ｐｐ．４０４４-４０５１
JPN6009021398; ＹＵ， Ｊ．， ｅｔ ａｌ．: 'Ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌ ｌｉｎｅｓ ｄｅｒｉｖｅｄ ｆｒｏｍ ｈｕｍａｎ ｓｏｍａｔｉｃ ｃｅｌｌｓ．' Ｓｃｉｅｎｃｅ ３１８, 2007, ｐｐ．１９１７-１９２０
Attorney, Agent or Firm:
Yasushi Okuhara