Title:
METHOD FOR PRODUCING CULTURE SUPERNATANT
Document Type and Number:
Japanese Patent JP2023094881
Kind Code:
A
Abstract:
To provide a novel method capable of easily improving the content of bioactive substances in culture supernatant.SOLUTION: A method for producing culture supernatant includes: a thawing process of thawing frozen cells; a first culture process of culturing the cells in a first culture medium; a removal process of removing culture supernatant obtained after the first culture process; a second culture process of further culturing the cells after the removal process in a second culture medium; and a recovery process of recovering culture supernatant obtained after the second culture process, where the first culture medium is a basic culture medium or a serum-free culture medium, and the second culture medium does not contain protein.SELECTED DRAWING: None
Inventors:
TERUNUMA YUTAKA
TERUNUMA ATSUSHI
SHIMIZU YOSHIHISA
ASHIBA KEISUKE
TERUNUMA ATSUSHI
SHIMIZU YOSHIHISA
ASHIBA KEISUKE
Application Number:
JP2021210450A
Publication Date:
July 06, 2023
Filing Date:
December 24, 2021
Export Citation:
Assignee:
BIOTHERAPY INST OF JAPAN INC
International Classes:
C12P21/02; C12N5/071
Attorney, Agent or Firm:
Patent Attorney Corporation Hiraki International Patent Office
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