To provide an industrial production process for L-α-oxyacid.

A cell body of a microorganism that belongs to Protaminobacter, Mycobacterium, Xanthobacter or Rhodococcus, and capable of selectively hydrolyzing L-α-oxyacid amide stereoselectively or cell body-treated product thereof is allowed to act on D,L-α-oxyacid amide represented by general formula: R- CH(OH)CONH (wherein R is a lower alkyl, a substituted lower alkyl, phenyl, a substituted phenyl) to produce the corresponding L-α-oxyacid.