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Title:
METHOD FOR PRODUCING NAIL TRICHOPHYTIC MODEL
Document Type and Number:
Japanese Patent JP2010142164
Kind Code:
A
Abstract:

To provide an in vitro nail trichophytic model high in reproducibility and separated from a living body.

The method for producing the nail trichophytic model comprises: sowing a preliminarily cultured, quantifiable fungus or its living body-constituting portion on the nail floor side of a non-infected nail picked up from an animal including human, and then culturing the sowed product in low temperature high humidity conditions for a long period without contacting with an aqueous carrier. The preliminarily cultured, quantifiable fungus or its living body-constituting portion is preferably a dispersion of a conidiospore, and the low temperature high humidity conditions preferably exit in a temperature range of 25-30°C and a humidity range of 90-100%.

COPYRIGHT: (C)2010,JPO&INPIT


Inventors:
KUBOTA NOBUO
NAGASAKA SAORI
OZAWA MICHIKO
Application Number:
JP2008323025A
Publication Date:
July 01, 2010
Filing Date:
December 19, 2008
Export Citation:
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Assignee:
POLA PHARMA INC
International Classes:
C12N1/14; C12M1/00; C12Q1/02; G01N33/15; G01N33/50
Domestic Patent References:
JP2007093584A2007-04-12
JP2008510537A2008-04-10
JP2001133449A2001-05-18
JP2007093584A2007-04-12
JP2007089565A2007-04-12
JP2010142149A2010-07-01
Foreign References:
WO2007102243A12007-09-13
WO2007102241A12007-09-13
WO2007076619A12007-07-12
Other References:
JPN6013030043; 和田初音(外5名): '爪白癬モデルを用いた薬効評価法の検討' 日本薬学会年会要旨集 Vol.127th, No.3, 20070305, p.134
JPN6009012128; Nakashima T,et al.: 'Experimental tinea unguium model to assess topical antifungal agents using the infected human nail w' J Infect Chemother(2002),Vol.8,p.331-335