Title:
リン脂質の製造方法
Document Type and Number:
Japanese Patent JP4216082
Kind Code:
B2
Abstract:
A new enzymatic process for preparing 1,2-diacylated phospholipids using an enzyme preparation possessing phospholipase activity towards acylation at the sn-1 and sn-2 sites in a microaqueous reaction system. More particularly, the 1,2-diacyl-phospholipids produced according to the esterification/transesterification process are obtainable in high yield and purity and carry identical desired carboxylic acid, preferably fatty acid, acyl groups at the sn-1 and sn-2 positions. The process involves esterification/transesterification (acylation) of a glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of the above mentioned appropriate enzyme preparation. The process of the invention further relates to a process for the production of 1-acyl-2-lyso-glycerophospholipid, preferably 2-lyso-PC by reacting glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of a sn-1 specific phospholipase (PLA1 or PLA1,2) and a solvent, in a microaqueous medium.
Inventors:
Bachelor, Sobuhi
Zabi, Rassan
Shulman, Avidor
Marl-Chime, story
Zabi, Rassan
Shulman, Avidor
Marl-Chime, story
Application Number:
JP2002587619A
Publication Date:
January 28, 2009
Filing Date:
May 02, 2002
Export Citation:
Assignee:
ENZYMOTEC LTD.
International Classes:
C12P9/00; C07F9/10; C12R1/66
Domestic Patent References:
| JP2000281688A | ||||
| JP9275980A | ||||
| JP3183480A | ||||
| JP6062850A | ||||
| JP7031472A |
Foreign References:
| WO2000056869A1 |
Other References:
青木淳賢,ホスファチジルセリン特異的ホスホリパーゼA1の構造と機能,脂質の分子生物学と病態生化学,共立出版,1999年 6月10日,第44巻,第8号,p.108-112,(蛋白質核酸酵素1999年6月号増刊)
山下哲、外1名,動物リゾホスホリパーゼの構造と機能,脂質の分子生物学と病態生化学,共立出版,1999年 6月10日,第44巻,第8号,p.113-121,(蛋白質核酸酵素1999年6月号増刊)
今堀和友、他1名 監修,生化学辞典(第3版),東京化学同人,1998年10月 8日,第3版,p.1312-1314,(ホスホリパーゼ関連の項目)
Process biochemistry. 1995, Vol.30, No.5, p.393-401
Journal of lipid research. 1985, Vol.26, No.12, p.1379-1388
山下哲、外1名,動物リゾホスホリパーゼの構造と機能,脂質の分子生物学と病態生化学,共立出版,1999年 6月10日,第44巻,第8号,p.113-121,(蛋白質核酸酵素1999年6月号増刊)
今堀和友、他1名 監修,生化学辞典(第3版),東京化学同人,1998年10月 8日,第3版,p.1312-1314,(ホスホリパーゼ関連の項目)
Process biochemistry. 1995, Vol.30, No.5, p.393-401
Journal of lipid research. 1985, Vol.26, No.12, p.1379-1388
Attorney, Agent or Firm:
Hideto Asamura
Hajime Asamura
Yukihiro Ikeda
Naganuma Akio
Hajime Asamura
Yukihiro Ikeda
Naganuma Akio