Title:
部位特異的組換えを用いた組換えウイルスの製造方法
Document Type and Number:
Japanese Patent JP4558328
Kind Code:
B2
Abstract:
Disclosed are the methods for producing recombinant viruses using in vitro site-specific recombination. In the present invention, linear viral genomic DNAs are digested to two segments, and are subjected to in vitro site-specific recombination with desired genomic materials flanked with site-specific recombinase sites. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system to generate hundreds or thousands of recombinant viruses.
Inventors:
Son, Howson
John, Nyon-Chi.
Scene, don-son
Kim, Kyung-Jin
Cho, Sung-Eun
Choi, Eun-Wook
John, Nyon-Chi.
Scene, don-son
Kim, Kyung-Jin
Cho, Sung-Eun
Choi, Eun-Wook
Application Number:
JP2003574812A
Publication Date:
October 06, 2010
Filing Date:
March 08, 2003
Export Citation:
Assignee:
BNR Science Company, Limited
International Classes:
C12N15/09; A61K35/76; A61K48/00; A61P43/00; C12N7/00; C12N7/01; C12N15/86; C12N15/861
Foreign References:
| US20020007051 |
Other References:
J. Virology, (1997), Vol. 71, No. 3, p. 1842-1849
Attorney, Agent or Firm:
Kenzo Hara International Patent Office