アルギン酸リアーゼ及び当該酵素を用いる不飽和ウロン酸単糖の製造方法

Title:

アルギン酸リアーゼ及び当該酵素を用いる不飽和ウロン酸単糖の製造方法

Document Type and Number:

Japanese Patent JP6954644

Kind Code:

B2

Abstract:

Alginate lyase activity is exhibited by the amino acid sequences (polypeptides) shown in SEQ ID No:1 and SEQ ID No:2. These polypeptides and their homology equivalents are used to produce 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) by contacting the polypeptide(s) with an alginate containing a uronic acid moiety and holding the mixture of the alginate and the polypeptide(s) at a temperature at which alginate lyase activity is exhibited.

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Inventors:

Toshiyuki Shibata
Hideo Miyake
Murase Yoshimitsu
Reiji Tanaka
Mori Tetsushi
Haruko Takeyama
Mami Takahashi

Application Number:

JP2018510583A

Publication Date:

October 27, 2021

Filing Date:

April 03, 2017

Export Citation:

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Assignee:

Mie University

International Classes:

C12N15/60; C12M1/00; C12N9/88; C12N15/63; C12P19/02; G01N30/02; G01N30/26; G01N30/72; G01N30/88

Domestic Patent References:

JP2015536641A

Foreign References:

WO2015074025A1

Other References:

Bioprocess and Biosystems Engineering,2014年,Vol.37,pp.2105-2111
NISHI, S., et al.,alginate lyase precursor [Loktanella cinnabarina LL-001].,Database DDBJ/EMBL/GenBank [online],2015年09月16日,Accession No. GAD57170,https://www.ncbi.nlm.nih.gov/protein/GAD57170
NISHI, S., et al.,oligo alginate lyase [Loktanella cinnabarina LL-001].,Database DDBJ/EMBL/GenBank [online],2015年09月16日,Accession No. GAD57174,https://www.ncbi.nlm.nih.gov/protein/GAD57174
第17回マリンバイオテクノロジー学会大会講演要旨集,2015年,pp.68 O-1-5

Attorney, Agent or Firm:

Yohei Kobayashi

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