Title:
METHOD FOR PURIFYING 168 KD PROTEIN FROM MYCOPLASMA PNEUMONIAE
Document Type and Number:
Japanese Patent JPH0236193
Kind Code:
A
Abstract:
PURPOSE: To obtain the subject high purity protein for diagnosing infectious diseases, etc., by a method in which all the cells of mycoplasma pneumoniae, after being treated with an amphoteric surfactant, are extraction-treated with a nonionic surfactant, and subjected to size exclusion chromatography.
CONSTITUTION: All the cells of mycoplasma pneumoniae is treated with an amphoteric surfactant such as 3-[(3-colamidepropyl)dimethylammonium]-1- propanesulfonate, 168 kD protein is extracted from the obtained pellets using a nonionic surfactant such as octyl-β-D-glucopyranoside (octylglucoside), and finally other impurities are removed by size exclusion chromatography to obtain the objective high purity protein.
Inventors:
BUORUFUGANGU BURETSUTO
Application Number:
JP6777589A
Publication Date:
February 06, 1990
Filing Date:
March 22, 1989
Export Citation:
Assignee:
BORUFUGANGU BURETSUTO
KUREMENSU FUFUTE
ENO YAAKOPUSU
KUREMENSU FUFUTE
ENO YAAKOPUSU
International Classes:
G01N33/569; C07K14/005; C07K14/195; C07K14/30; C07K14/41; C09K23/22; C09K23/56; C12P21/00; C12R1/00; (IPC1-7): C07K3/12; C07K3/20; C07K15/04; C12P21/00; G01N33/569
Attorney, Agent or Firm:
Chika Takagi (2 outside)
Next Patent: IMMUNOACTIVE POLYPEPTIDE