Title:
METHOD FOR PURIFYING BIOMOLECULES USING APTAMER
Document Type and Number:
Japanese Patent JP2021101714
Kind Code:
A
Abstract:
To provide methods for purifying a specific biomolecule from a sample of a patient with a disease and identifying a biomarker for a specific disease by using an oligonucleotide aptamer that binds to a specific biomolecule associated with the disease.SOLUTION: The present invention describes a method of using magnetically assisted fast aptamer selection (MARAS) to select an aptamer that has a high binding affinity for a biomolecule associated with a certain disease, from a nucleic acid library, and has no binding affinity for other biomolecules associated with no disease. On the other hand, as a capture ligand for purifying a specific biomolecule associated with a disease from a sample of a patient with a disease, a method of using the obtained aptamer is also presented. Furthermore, the invention describes using the obtained aptamer, as a capture ligand for purifying biomolecules with a specific binding affinity range by applying a vibrating magnetic field range as a virtual filter.SELECTED DRAWING: Figure 2
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Inventors:
HONG CHIN-YIH
HORNG HERNG-ER
HORNG HERNG-ER
Application Number:
JP2021043621A
Publication Date:
July 15, 2021
Filing Date:
March 17, 2021
Export Citation:
Assignee:
HONG CHIN YIH
HORNG HERNG ER
HORNG HERNG ER
International Classes:
B03B5/28; C12N15/115; B03B7/00; B03C1/00; B03C1/01; B03C1/23; B03C1/28; B03C5/00; G01N33/53
Domestic Patent References:
| JP2015084775A | 2015-05-07 |
Foreign References:
| US20180095078A1 | 2018-04-05 |
Other References:
ACS COMB SCI, (2014), VOL.16, PP.321-327, JPN6020019585, ISSN: 0004768771
J MATER CHEM B, (2014), VOL.2, PP.4114-4121, JPN6020019587, ISSN: 0004768772
J MATER CHEM B, (2014), VOL.2, PP.4114-4121, JPN6020019587, ISSN: 0004768772
Attorney, Agent or Firm:
Kenji Sugimura
Mitsutsugu Sugimura
Mutsuya Yamamoto
Mitsutsugu Sugimura
Mutsuya Yamamoto