Title:
試料中に存在する鋳型核酸の定量方法
Document Type and Number:
Japanese Patent JP4521581
Kind Code:
B2
Abstract:
A method for determining the amount of template nucleic acid present in a sample comprising the steps of: I) bringing into association with the sample all the components necessary for nucleic acid amplification, and all the components necessary for a bioluminescence assay for nucleic acid amplification and subsequently: ii) performing the nucleic acid amplification, iii) monitoring the intensity of light output from the bioluminescence assay; and iv) determining the amount of template nucleic acid present in the sample.
Inventors:
LAURENCE C. TISI
JAMES A. H. MURRAY
JAMES A. H. MURRAY
Application Number:
JP2006500210A
Publication Date:
August 11, 2010
Filing Date:
January 14, 2004
Export Citation:
Assignee:
LUMORA LIMITED
International Classes:
C12Q1/68; C12M1/00; C12N15/00; C12N15/09; C12Q1/26; C12Q1/48; C12Q1/6851; G01N21/76
Foreign References:
| WO2001042496A1 | ||||
| WO2002064830A1 |
Other References:
Analytical Biochemistry, 288(2001) p.28-38
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Kenji Tomita
Mina Shiga
Masayuki Nakagawa
Fumio Inai
Hiroshi Yamazaki
Kenji Tomita
Mina Shiga
Masayuki Nakagawa
Fumio Inai