To obtain a method for quantitatively analyzing a target compound of which content in an organism sample is extremely small, by a high-speed liquid chromatography/mass spectrometer system.
The inner diameter of the column for a high-speed liquid chromatography is set to 1.0-4.6mm, the length is set to 1-25cm, and the particle diameter of an inverse-phase system filler in the column is 3-10μm. A fixed amount of target compound which is equal to or more than 0.1pg/ml and less than 10pg/ml is injected into a mass spectrograph and an optimized product ion is set to a take-in width which is 1 mass unit to 2 mass units for measurement, thus achieving, with an improved reproducibility, a relative standard deviation of 20% or less for a quantitative lower limit which is 0.1pg to less than 10pg when the content of the quantitative target compound in a sample is less than 1μg/ml.
MATSUZAKI KAZUE
MIZOOKU YASUO
SAWA UTAJI
KITAHARA HAJIME
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