Title:
METHOD FOR RECOVERING NUCLEIC ACID
Document Type and Number:
Japanese Patent JP3644641
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To efficiently recover ribonucleic acid and/or deoxyribonucleic acid from a sample containing a sample containing ribonucleic acid and deoxyribonucleic acid.
SOLUTION: The method for recovering nucleic acid comprises bringing a low-temperature eluate into contact with a nucleic acid-capturing substance having captured ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). The recovery ratio of RNA and DNA by the eluate changes with the temperature of the eluate and the low-temperature eluate has greatly larger recovery ratio of DNA than that of RNA. Consequently, DNA is efficiently recovered from the sample containing DNA and RNA.
Inventors:
Tomoya Sakurai
Toshiaki Yokobayashi
Toshiaki Yokobayashi
Application Number:
JP2003060983A
Publication Date:
May 11, 2005
Filing Date:
March 07, 2003
Export Citation:
Assignee:
Hitachi High-Technologies Corporation
International Classes:
C12N15/09; C12N15/00; C12N15/10; (IPC1-7): C12N15/00
Domestic Patent References:
| JP11507544A | ||||
| JP9327291A |
Attorney, Agent or Firm:
Yusuke Hiraki